Protective effect of UDCA, Resveratrol and NAC on Non-Alcoholic Fatty Liver Disease (NAFLD)

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Protective effect of ursodeoxycholic acid, resveratrol, and N-acetylcysteine on nonalcoholic fatty liver disease in rats.

Abstract

CONTEXT:

Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease. Resveratrol (RSV) and N-acetylcysteine (NAC) are safe representatives of natural and synthetic antioxidants, respectively.

OBJECTIVE:

The objective of this study was to evaluate protective effects of RSV and NAC, compared with ursodeoxycholic acid (UDCA), on experimental NAFLD.

MATERIALS AND METHODS:

NAFLD was induced by feeding rats a methionine choline-deficient diet (MCDD) for four cycles, each of 4?d of MCDD feeding and 3?d of fasting. Animals were divided into normal control, steatosis control, and five treatment groups, receiving UDCA (25?mg/kg/d), RSV (10?mg/kg/d), NAC (20?mg/kg/d), UDCA?+?RSV, and UDCA?+?NAC orally for 28?d. Liver integrity markers (liver index and serum transaminases), serum tumor necrosis factor-? (TNF-?), glucose, albumin, renal functions (urea, creatinine), lipid profile (total cholesterol; TC, triglycerides, high density lipoproteins, low density lipoproteins; LDL-C, very low density lipoproteins, leptin), and oxidative stress markers (hepatic malondialdehyde; MDA, glutathione; GSH, glutathione-S-transferase; GST) were measured using automatic analyzer, colorimetric kits, and ELISA kits, supported by a liver histopathological study.

RESULTS:

RSV and NAC administration significantly improved liver index (RSV only), alanine transaminase (52, 52%), TNF-? (70, 70%), glucose (69, 80%), albumin (122, 114%), MDA (55, 63%), GSH (160, 152%), GST (84, 84%), TC (86, 86%), LDL-C (83, 81%), and leptin (59, 70%) levels compared with steatosis control values. A combination of RSV or NAC with UDCA seems to ameliorate their effects.

DISCUSSION AND CONCLUSION:

RSV and NAC are effective on NAFLD through antioxidant, anti-inflammatory, and lipid-lowering potentials, where as RSV seems better than UDCA or NAC.

Reference:

Ali MH, Messiha BA, Abdel-Latif HA. Protective effect of ursodeoxycholic acid, resveratrol, and N-acetylcysteine on nonalcoholic fatty liver disease in rats. Pharm Biol. 2016 Jul;54(7):1198-208. doi: 10.3109/13880209.2015.1060247. Epub 2015 Jul 1. PubMed PMID: 26134756.

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